Trans fatty acids may be involved in atherosclerotic vascular diseases. We investigated the incorporation of dietary trans fatty acids and oleic acid into the serum triglycerides (TG), cholesterol esters (CE), and phospholipids (PL). Fourteen healthy female volunteers, aged 23.2+/-3.1 yr (mean+/-SD), body mass index 20.8+/-2.1 kg/m2 participated in this study. All subjects consumed both a trans fatty acid-enriched diet (TRANS diet) and an oleic acid-enriched diet (OLEIC diet) for 4 wk according to a randomized crossover design. Both experimental diet periods were preceded by consumption of a baseline diet for 2 wk which supplied 37% of total energy (E%) as fat: 18 E% from saturated fatty acids (SFA), 12 E% from monounsaturated fatty acids, and 6 E% from polyunsaturated fatty acids. Five E% of the SFA in the baseline diet was replaced by trans fatty acids (18:1t and 18:2c,t + 18:2t,t, where c is cis and t is trans) in the TRANS diet and by oleic acid (18:1n-9) in the OLEIC diet. After the TRANS diet, the proportions of 18:1t and 18:2t increased (P<0.001) in all serum lipid fractions analyzed. The increase of 18:1t in TG and PL (1.80+/-0.28 vs. 5.26+/-1.40; 1.07+/-0.34 vs. 3.39+/-0.76 mol% of total fatty acids, respectively) was markedly higher than that in CE (0.44+/-0.07 vs. 0.92+/-0.26), whereas that of 18:2t was nearly the same in all three fractions. The proportions of palmitic, stearic, arachidonic, and eicosapentaenoic acids in TG, CE, and PL and that of oleic acid in TG and CE were decreased when compared with the baseline value. In contrast, the proportion of palmitoleic acid in TG and PL and that of linoleic acid in PL increased on the TRANS diet. After consumption of the OLEIC diet, the proportion of oleic acid increased in all three lipid fractions analyzed, and the percentage increase was nearly the same in all fractions. In contrast, the proportions of 18:1t in TG and PL and 18:2t in TG and CE decreased when compared with the baseline value. In conclusion, a moderate increase in dietary trans fatty acids resulted in a marked incorporation into serum lipids and decreased the conversion of linoleic acid to its more unsaturated long-chain metabolites. Analysis of 18:1t from serum TG and PL seems to reflect reliably the dietary intake of this fatty acid.