Abstract
Recent findings suggest that use of colistin as a last resort antibiotic is seriously threatened by the rise of a new plasmid mediated mechanism of resistance (MCR-1). This work identifies, for the first time in Southern Europe, the gene mcr-1 in nine strains from farm animals (poultry and swine) corresponding to five Escherichia coli and four Salmonella enterica, among which three belong to serovar Typhimurium and one to Rissen. The MCR-1 was found encoded by a plasmid highly mobilizable by conjugation to the E. coli J53 strain. Two E. coli strains carried two determinants, mcr-1 plus pmrA or pmrB mutations, known to confer colistin resistance.
Keywords:
Colistin resistance; E. coli; Plasmid; PmrAB; S. enterica; mcr-1.
Copyright © 2016 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Colistin / pharmacology*
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Drug Resistance, Bacterial*
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Escherichia coli / drug effects*
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Escherichia coli / physiology
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Escherichia coli Infections / epidemiology
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary
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Poultry Diseases / epidemiology
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Poultry Diseases / microbiology
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Salmonella Infections, Animal / epidemiology
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Salmonella Infections, Animal / microbiology
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Salmonella enterica / drug effects*
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Salmonella enterica / physiology
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Salmonella typhimurium / drug effects
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Salmonella typhimurium / physiology
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Sequence Analysis, DNA / veterinary
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Spain / epidemiology
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Swine
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Swine Diseases / epidemiology
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Swine Diseases / microbiology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Colistin