Elsevier

Journal of Affective Disorders

Volume 207, 1 January 2017, Pages 300-304
Journal of Affective Disorders

Research paper
Prevotella and Klebsiella proportions in fecal microbial communities are potential characteristic parameters for patients with major depressive disorder

https://doi.org/10.1016/j.jad.2016.09.051Get rights and content

Highlights

  • The changes of the Prevotella and Klebsiella proportion in fecal microbial communities were consistent with Hamilton depression rating scale in major depression disorder patients.

  • Specific genera continuous observation can be used in the diagnosis and therapeutic monitoring of major depression disorders.

Abstract

Background

The diagnosis of major depression disorder (MDD) and other mental disorders were depended on some subjective survey scales. There are confirmed relationship between the gut flora and the mental states of MDD patients.

Methods

The V3–V4 region of the 16S rRNA gene was extracted from the fecal microbial communities in MDD patients, PCR amplified and sequenced on the Illumina Miseq platform.

Results

More phylum Firmicutes, less Bacteroidetes, and more genus Prevotella, Klebsiella, Streptococcus and Clostridium XI were found in MDD patients. The changes of the proportion of Prevotella and Klebsiella were consistent with Hamilton depression rating scale.

Limitations

The conclusion was limited by small sample sizes and potential uncontrollable influence factors on fecal microbiota.

Discussion

Prevotella and Klebsiella proportion in fecal microbial communities should be concerned in the diagnosis and therapeutic monitoring of MDD in future.

Section snippets

Instruction

Major depressive disorder is one of the major and common psychiatric disorders with high rates of self-harm and suicide attempts. The real causes and pathogenesis of major depressive disorder are not well understood (Hegerl et al., 2013). A diverse contribution of genetic, neurochemical and environmental factors are involved in the onset and progression of depression (Wang et al., 2015). The bidirectional interactions between the central nervous system, the enteric nervous system, and the

Ethics statement

The institutional Review Board (IRB) at Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine approved the study. Patients who expressed interest in joining this study were introduced the details and signed the written consent forms.

Subject recruitment and study procedure

Sixty MDD patients were recruited in this study, and another 60 people were selected as control. The basic enrollment criteria of the patient recruitment are: (1) age 20–85 years; (2) no usage of any type of antibiotic, antifungal

Subject characteristics

Ten MDD patients performed the study procedure completely, that means total 30 patients’ stool specimen were collected for metagenomic sequencing in this study. Another 10 control stool samples were randomly selected from the 60 subjects. The common characteristics of 10 MDD patients and control subjects were shown in Table 1.

Major bacterial organism in subjects of MDD and control groups

All MDD patients received the regular treatment in this study. The major bacterial organism in subjects of MDD (3 visits) and control groups were calculated in phylum

Discussion

Intestinal microbiota constitutes a symbiotic ecosystem that keeps homeostatic balance inside the human body. However, this equilibrium can be disrupted by pathological conditions interfering with intestinal physiology (Kang et al., 2013). There are complicated correlation between the components of gut flora and several diseases, includes infectious diseases and non infectious disease. However, the pattern of gut flora is affected by many factors such as the diet, race, geography, local

Conclusions

The proportion changes of prevotella and klebsiella in fecal flora could be useful parameters for the laboratory diagnosis and treatment evaluation in MDD patients.

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