PT - JOURNAL ARTICLE AU - Sophie Mineau AU - Robert Kozak AU - Melissa Kissoon AU - Aimee Paterson AU - Anthony Oppedisano AU - Firas Douri AU - Kate Gogan AU - Barbara M. Willey AU - Allison McGeer AU - Susan M. Poutanen TI - Emerging antimicrobial resistance among <em>Escherichia coli</em> strains in bloodstream infections in Toronto, 2006–2016: a retrospective cohort study AID - 10.9778/cmajo.20180039 DP - 2018 Oct 01 TA - CMAJ Open PG - E580--E586 VI - 6 IP - 4 4099 - http://www.cmajopen.ca/content/6/4/E580.short 4100 - http://www.cmajopen.ca/content/6/4/E580.full SO - CMAJ2018 Oct 01; 6 AB - Background: Enterobacteriaceae that produce extended-spectrum β-lactamase (ESBL) have emerged as a serious threat, with variable rates depending on geographic region. We determined the prevalence of ESBL-producing Escherichia coli, Klebsiella pneumoniae, K. oxytoca and Proteus mirabilis in bloodstream infections in Toronto from 2006 through 2016.Methods: All patients with E. coli, K. pneumoniae, K. oxytoca and P. mirabilis isolated from blood in a tertiary care microbiology laboratory in Toronto between 2006 and 2016 (1 isolate per species per patient per year) were included in this retrospective cohort study. Organisms were identified by conventional methods, and susceptibility testing was performed according to Clinical and Laboratory Standards Institute standards. Screening for ESBL and phenotypic confirmatory testing were done with a modified Clinical and Laboratory Standards Institute method. ST131 clonal type was determined by means of an established protocol.Results: The proportion of ESBL-producing E. coli isolates increased significantly between 2006 and 2016, from 6.4% (19/296) to 17.3% (89/513) (p &lt; 0.001). This trend was seen in both intensive care units and emergency departments. Concurrently, the proportion of ST131 among ESBL-producing E. coli also increased significantly, from 31.6% (6/19) in 2006 to 73.0% (65/89) in 2016 (p = 0.03). Among ESBL-producing E. coli, significant resistance was noted to multiple antimicrobial classes. Comparable increases in the proportion of ESBL-producing K. pneumoniae, K. oxytoca and P. mirabilis were not noted.Interpretation: We observed a significant increase in the proportion of ESBL-producing E. coli in bloodstream infections in Toronto temporally correlated with an increase in the ST131 clonal type. Recognition of this dramatic rise is important to inform empiric antibiotic treatment.